Fig. 3

Dried inactivated yeast interfering RNA tablets induce significant larval death. Dried inactivated yeast interfering RNA larvicide tablets (a; penny shown for scale) were prepared and fed to 20 larvae. Significant larval death was observed in larvae fed with yeast expressing shRNA hairpins corresponding to the Sac1.1, lrc.51, and otk.16 target sequences as compared to larvae fed control yeast interfering RNA tablets (b). Data were compiled from three biological replicate experiments (total n = 240 larvae/condition; see “Methods” for details) and analysed by ANOVA with Tukey’s multiple comparison test. ***p < 0.001 in comparison to control-fed larvae; error bars denote SEMs