Fig. 5

Neural defects observed in larvae treated with yeast interfering RNA larvicides Sac1.1, lrc.51, and otk.16. L4 larval brains were labeled with mAb3C11 (white in a1–d1; red in a2–d2), which labels expression of Synapsin, a marker for the neuropil and synaptic active zones. TO-PRO was used to counter-stain nuclei in the brain (blue in a2–d2). The brains of larvae fed with yeast expressing shRNAs Sac1.1 (b1, b2), lrc.51 (c1, c2), and otk.16 (d1, d2) yeast interfering RNA tablets show loss of staining in the synaptic neuropil regions when compared with animals fed with control yeast (a1, a2). Three biological replicate experiments were performed. The data shown are representative of the results from 25 brains evaluated per condition. LAL, larval antennal lobe; OF, olfactory foramen; OL, optic lobe; SOG, sub-oesophageal ganglion; SuEG, supra-oesophageal ganglion