Fig. 2

Differential reactivity of antibodies with air-dried vs. methanol fixed sporozoites. Various concentrations of intact sporozoites (a, b) or sporozoite lysates (c, d) were plated and air-dried or methanol fixed. Plates were then probed with either a CSP-specific mAb (2A10) (a, c) or pooled sera from RAS immune subjects (b, d). The dashed lines indicate the background activity [ELISA signal obtained with either the control mouse mAb 1D9 (OD < 0.05) or pre-immune serum pool (OD < 0.12)]. Asterisks indicate statistical significance (p < 0.05, paired T test)