Fig. 1

Monitoring of the UDP-GlcNAc content in Plasmodium falciparum. UDP-GlcNAc is the end-product of the hexosamine biosynthetic pathway (a). Among other GlcNAc transferases, UDP-GlcNAc is the substrate of OGT (adapted from Aquino-Gil et al. [58]). HK/GK, hexokinase/glucokinase; GPI, glucose-6-phosphate isomerase; GFAT, glutamine: fructose-6-phosphate amidotransferase; GNPNAT1, GlcNH₂-6-phosphate N-acetyltransferase 1; AGM1, phospho-GlcNAc mutase 1; UAP, UDP-GlcNAc pyrophosphorylase; OGT, O-GlcNAc transferase. Late trophozoite UDP-GlcNAc contents were analysed by using a FPLC system (AKTA purifier; ProPAC-PA1 column; detection carried out at 256 nm) as described in the materials session (blue line) (b). A co-injection of the sample with 0.5 nmol UDP-GlcNAc is shown in red. Increasing amounts of standard UDP-GlcNAc were injected for calibration (top insert). The quantity of UDP-GlcNAc was normalized for 1 mg of proteins for comparison with MRC5 cells (bottom insert). c Uninfected erythrocytes were analysed alone according to their UDP-GlcNAc content or co-injected with 1 nmol of UDP-GlcNAc. d Total proteins from P. falciparum and erythrocytes were run using SDSPAGE (right panel). The gel was stained with brilliant blue