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Fig. 3 | Malaria Journal

Fig. 3

From: In silico epitope mapping and experimental evaluation of the Merozoite Adhesive Erythrocytic Binding Protein (MAEBL) as a malaria vaccine candidate

Fig. 3

Plasmodium yoelii MAEBL antisera pan-reactivity against human malaria parasites. a rPyM2MAEBL antibodies recognize Plasmodium falciparum (3D7 strain) schizonts. b rPyM2MAEBL antibodies also recognize Plasmodium vivax schizonts. Sera pool from the 4th dose of animals immunized with rPyM2MAEBL, prime-boost regimen or of mice injected with adjuvant only (CFA/IFA) were used at a dilution of 1:50 in indirect immunofluorescence assays against P. falciparum or P. vivax schizonts. Sera from mice immunized with rPyM2-MAEBL or heterologous prime-boost system inhibit invasion by c P. falciparum to normocytes and d P. vivax to reticulocytes. Schizonts and target cells were cultured in vitro in the presence of sera pool from the fourth dose of immunized mice diluted 1:50. As a positive control for inhibition in P. vivax assays anti-Duffy polyclonal sera was used in 1:50 dilution. In P. falciparum, E64 protease inhibitor (Sigma) was used as positive control of inhibition. For both species sera from mice injected with CFA/IFA were used as a negative control. The parasitaemia was determined by counting at least 1000 red blood cells. The results are expressed as percentage inhibition compared to the control without sera

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Malaria Journal

ISSN: 1475-2875

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