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Table 2 Percentage of gametocytes produced from RKL-9 and JDP-8

From: Methylene blue induced morphological deformations in Plasmodium falciparum gametocytes: implications for transmission-blocking

Isolate

ADuration of asexual culture before induction (in months)

BNumber of cryopreservation events before induction

0.5

6.5

1

4

RKL-9

2.24 ± 0.21

2.15 ± 0.42

2.40 ± 0.19

2.58 ± 0.46

JDP-8

2.02 ± 0.22

2.21 ± 0.23

2.28 ± 0.56

2.00 ± 0.39

  1. Comparison of % gametocytaemia. AAt the interval of 6 months (expressed as mean ± SD of three separate induction experiments). BAfter three cryopreservation events (expressed as mean ± SD of four separate induction experiments). No significant difference (p > 0.05) in percentage gametocytaemia after induction was observed in RKL-9 and JDP-8 for both the experiments A(effect of duration of parasites in asexual culture) and B(effect of number of cryopreservation events). Percentage gametocytaemia calculations are per 5000 RBCs