Stabilization of RDT target antigens present in dried Plasmodium falciparum-infected samples for validating malaria rapid diagnostic tests at the point of care

Table 3 Baseline stability of Plasmodium HRP2, pLDH, and aldolase in non-stabilized samples (study positive controls) during the 53 weeks of analysis

Sample type	Parasite concentration	Malaria RDTs	HRP 2		pLDH and aldolase
Sample type	Parasite concentration	Malaria RDTs	Reactivity (in weeks)	Percentage stability	Reactivity (in weeks)	Percentage stability
Culture Sample	2000 P/µL	SD Bioline	24	45.28	12	22.64
		First Response	24	45.28	12	22.64
		BinaxNOW	24	45.28	18	33.96
	500 P/µL	SD Bioline	24	45.28	6	11.32
		First Response	24	45.28	6	11.32
		BinaxNOW	24	45.28	2	3.77
	200 P/µL	SD Bioline	24	45.28	0	0.00
		First Response	24	45.28	0	0.00
		BinaxNOW	24	45.28	0	0.00
Patient Sample	2000 P/µL	SD Bioline	24	45.28	12	22.64
		First Response	21	39.62	12	22.64
		BinaxNOW	24	45.28	2	3.77
	500 P/µL	SD Bioline	24	45.28	4	7.55
		First Response	21	39.62	2	3.77
		BinaxNOW	21	39.62	2	3.77
	200 P/µL	SD Bioline	24	45.28	0	0.00
		First Response	24	45.28	0	0.00
		BinaxNOW	24	45.28	0	0.00

P/µL, Parasites per microliter; RDTs, rapid diagnostic Tests; pLDH, Plasmodium lactate dehydrogenase; HRP2, histidine rich protein II

ISSN: 1475-2875