Fig. 6

Intracellular iron stores in the spleen. Perl’s Prussian Blue iron stain of the spleen from an iron deficient mouse that received intravenous ferric carboxymaltose. Iron deposits within macrophages appear bright blue. Five spleens of surviving animals randomly selected from every group (n = 25) were examined at the end of the experiment (Day 22), when the mice were killed. All iron-replete mice and ferric carboxymaltose-supplemented iron-deficient mice had intracellular iron stores in the spleen, whereas this was not seen in any of the carboxymaltose- or ferrous sulfate-treated mice (not shown) except scarce amounts of iron in 1 of 5 iron-deficient mice that received carboxymaltose Day 7 to Day 9 post-infection