Fig. 1

Anopheles gambiae species-diagnostic PCR gel electrophoresis showing the amplification by other anopheline species. Lanes 1 and 26: 100 bp DNA Ladder; lanes 2–5: positive controls for An. arabiensis; An. gambiae; An. merus and An. quadriannulatus; lanes 6, 7: negative controls for DNA extraction and PCR master mix; lane 10: An. rufipes; lanes 11–21: An. listeri; lanes 22, 23: An. squamosus; lanes 24, 25 contained no specimens