Table 1 Anopheline species collected from five African countries on which different species-diagnostic PCR analyses were conducted
| Country | Province/site | GPS coordinates | Morphological ID | Molecular ID using An. gambiae complex PCR | Molecular ID using An. funestus group PCR | Molecular ID using An. funestus-like PCR | Molecular ID using An. rivulorum-like PCR |
|---|---|---|---|---|---|---|---|
| Botswana | Xakanaka |
19°25′32.0″S 23°38′46.0″E | An. wellcomei group (n = 1) | – | – | – | – |
| Guinea Conakry | Siguiri |
11°33′58.1″N 09°25′39.1″W | An. rufipes (n = 8) | – | An. leesoni (5) | – | – |
| Mali | Yanfolila |
11°10′40.8″N 8°09′10.8″W | An. rufipes (n = 10) | – |
An. rivulorum (1) An. leesoni (1) | – | – |
| Namibia | Katima Mulilo |
17°29′48.1″S 24°15′51.5″E | An. squamosus (n = 2) | An. gambiae (2) | – | – | – |
| South Africa | Kwazulu-Natal |
27°26′3.59″S 32°11′25.11″E | An. rufipes (n = 19) | – | – | – | – |
| Gauteng |
25°59′23.8″S 27°54′00.2″E | An. crypticus (n = 17) | – | – | – | – | |
|
An. marshallii
(n = 1) | – | – | – | – | |||
| Mpumalanga |
25°38′15.4″S 31°43′20.6″E | An. coustani (n = 6) | – | – | – | – | |
| An. tenebrosus (n = 1) | – | – | – | – | |||
| An. pretoriensis (n = 20) | – | – | – | – | |||
| An. rufipes (n = 9) | An. gambiae/An. merus (1) | – | – | – | |||
| An. maculipalpis (n = 10) | – | – | – | – | |||
| Limpopo |
23°55′ 12.00″S 31°15′ 36.00″E | An. maculipalpis (n = 1) | – | – | – | – | |
| An. rhodesiensis (n = 1) | – | An. leesoni (1) | – | – | |||
| An. listeri (n = 12) |
An. gambiae (3) An. merus (2) | – | – | – | |||
| Kruger National Park |
23°59′18.2″S 31°33′16.9″E | An. pretoriensis (n = 26) |
An. arabiensis (1) An. gambiae (3) |
An. funestus (3) An. vaneedeni (1) | – | – | |
| An. rufipes (n = 6) |
An. arabiensis (3) An. gambiae (2) | – | – |