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Fig. 2 | Malaria Journal

Fig. 2

From: A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax

Fig. 2

Identification of Plasmodium sporozoite-associated receptors on immortalized hepatocyte-like cells (imHCs). The expression of Plasmodium sporozoite-associated receptors (CD81, EphA2 and SR-BI) on imHCs and HC-04 cells was determined using immunofluorescent staining. Prior to analysis, the hepatocytes were grown in MEM/F12 medium containing 10% fetal bovine serum until confluence was reached. The expression of CD81 (a), EphA2 (b) and SR-BI (c) on imHCs and HC-04 cells was compared (d, e, f, respectively). Hepatocyte nuclei were stained with Hoechst 33342 dye. Fluorescence images were captured and analysed using an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. Cells expressing CD81, EphA2 or SR-BI were quantified from 15 randomly selected image fields (total number of analysed cells > 2000) (g, h, i, respectively). Bar graph shows the mean percentage of positively stained cells. Error bars depict standard deviations. ****p < 0.0001, Student’s t test

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