Fig. 6

In vitro growth of intra-hepatocytic Plasmodium vivax in immortalized hepatocyte-like cell (imHC) culture. Plasmodium vivax sporozoites were co-cultured with imHCs for 4, 7, and 10 days. Exoerythocytic forms (EEs) were visualized in an indirect immunofluorescence assay using antibodies against parasite UIS4, ACP and H3K9Ac. The hepatocyte nuclei were stained using DAPI. The sizes of individual EEs from duplicate wells were measured at each time point and plotted (a), and representative immunofluorescence images of intracellular P. vivax EEs on day 10 (b) and 14 post-infection (c) are shown. Scale bar = 10 μm