Genetic variants of RNASE3 (ECP) and susceptibility to severe malaria in Senegalese population

Malaria Journal

Table 4 Single Nucleotide Polymorphism (SNP) and association analysis with susceptibility to severe malaria (SM)

NCBI dbSNP number	Phénotype	MAF				Nominal p values for statistical test (additive models)
NCBI dbSNP number	Phénotype	SM	UM	CTR	All	SM vs. UM	UM vs. CTR	SM vs. CTR
A. Hb S and Hb C polymorphisms and association with SM
rs334 A > T OR (95% CI)	HbS	0.026	0.069	0.035	0.050	0.033 2.67 (2.01–3.5)	0.257	0.446
rs33930165 G > A OR (95% CI)	HbC	0.000	0.009	0.029	0.011	0.258	0.1661	0.0084 1.67 (0.99–3.42)
rs713040 C > T OR (95% CI)		0.107	0.068	0.119	0.099	0.097	0.029 *2.33* (*1.55–7.33*)	0.660

RNASE3 SNPs		Nominal p values for statistical test
Position (NCBI Ids)	Associated allele	UM vs. CTR		SM vs. CTR		SM vs. UM		SM vs. UM + CTR
Position (NCBI Ids)	Associated allele	ADD	D/R	ADD	D/R	ADD	D/R	ADD	D/R
B. Association analysis with susceptibility to severe malaria under Hb polymorphisms corrections
Promoter −550G/A (rs2284954)	A	0.30		0.81		0.71		0.84
Promoter −399T/C (rs147413155)	C	0.36		0.28		0.55		0.23
Promoter −38C/A (rs2233859)	A	0.38		0.29		0. 98		0.47
Coding +371_G/C (rs2073342) OR (95% CI)	G	0.08 1.8 (0.35–0.91)	0.01 (D) 3.9 (1.29–11.7)	0.05 1.78 (0.99–3.1)	0.08 (D) 2.18 (0.88–5.36)	0.86		0.17
3′utr + 499_G/C (rs2233860) OR (95% CI)	C	0.52		0.05 1.46 (1.21–2.09)	0.01 (D) 1.30 (1.11–1.79)	0.003 1.43 (1.20–1.92)	0.01 (D) 1.28 (1.10–1.76)	0.001 1.50 (1.30–1.84)	0.004 (D) 1.29 (1.21–1.74)
3′utr + 577_A/T (rs8019343)	T	0.66		0.16		0.17		0.06 1.83 (0.96–3.05)	0.07(D) 1.98 (0.92–4.32)

For each polymorphism, the p values for statistical tests were indicated. Statistical tests used were logistic regression analysis. (A) Association analysis for HB polymorphisms by comparison of SM vs Controls group B) RNASE3 polymorphisms association analysis adjusted for HbS polymorphisms (rs334 A > T) (B). The associated allele of polymorphism is given. The calculation modes for logistic regression test are indicated using different models (ADD additive, D Dominant, R Recessive modes). Borderline (0.05 ≤ p ≤ 0.1) and significant (0 ≤ p ≤ 0.05) p values are in bold. The p values for the dominant/recessive mode were showed only when lower than 0.1. (* Bonferroni corrections were not performed on p values). The OR (odds ratio) and CI (Confidence intervals) were showed when p values are significant or in borderline and were reprented in italic

ISSN: 1475-2875