Table 2 A set of 12 microsatellite markers that distinguishes parents and recombinant progeny of four Plasmodium falciparum crosses
Marker | Chromosome | Location (kB) | Microsatellite peak size (base pairs) | Forward primer | Reverse primer | Microsatellite repeat sequence-including F/R primers (3D7) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
GB4 | 803 | Dd2 | HB3 | 3D7 | 7G8 | ||||||
C3M64 | 3 | 477 | 109 | 174 | 174 | 184 | 198 | 188 | AAAGAATAAGAATAGGAATCA | AACAAAACATGCTTATCTAAA | AAAGAATAAGAATAGGAATCATATATATATACATACATATACACACATATATATATATATATACATATATATATACACACATATATATATATACACATATATATATATATATATATATATATATATATATATATATATATATATATATATACATATTAATAAAGATTCATTTTATATTATTTAGATAAGCATGTTTTGTT |
C3M54 | 3 | 909 | 215 | 264 | 234 | 241 | 238 | 266 | AATATAATCATAAAGTCGTAC | CTAAGAGAAAAAATGGGTAT | AATATAATCATAAAGTCGTACTTTTTTTTTTTTATTTTATATAATAAATAAATATAAAATAAATAAAATATTTATATATATATATATATATATAAATGTTATTATATATATATATATATATATATATATATATATATTTTTATAATATATATAATAACTGGATTCTATTTTTTTTTAGAGTAATATATATATATATATATATAATTATATTTTTATATAATACCCATTTTTTCTCTTAG |
C4M30 | 4 | 1087 | 164 | 189 | 165 | 180 | 193 | 167 | ATTGATGCTTTGTCTAATTAG | ATGACAAAACATGGTATGTA | ATTGATGCTTTGTCTAATTAGAGTATATGATATATATATATATATAAAAAAAAAAAAATAAAAAATAAAATAAATAAAAAATAAAAAAAATAAAAAATAAAAAATAAAATATATATATATATATATATATATATATATATATATATATATATATTTTTTTTTATATATTCATATACATACCATGTTTTGTCAT |
B5M3 | 5 | 494 | 120 | 114 | 123 | 127 | 121 | 133 | AAGTTATGATGAATTTGATTC | TTATTATCATCATCACCCATC | AAGTTATGATGAATTTGATTCTAAAATATCAAAATATCCAACAAAAGTAGGAAATGAAGGGAAAAAATCAAAAGATAATAATAATAATAATAATAATAATATGATGGGTGATGATGATAATAA |
TAA81 | 5 | 1214 | 121 | 115 | 121 | 127 | 118 | 115 | TTTCACACAACACAGGATT | TGGACAAATGGGAAAGGATA | TTTCACACAACACAGGATTATTACCATCACCATTAATGTTATCATTCCTTGTATTATTATTATTATTATTATTATTATTATGTTTGATTTGGTTATCATCTTTATCCTTTCCCATTTGTCCA |
TAA87 | 6 | 374 | 94 | 109 | 107 | 104 | 95 | 110 | ACATGTTCATATTACTCAC | AATGGCAACACCATTCAAC | ACATGTTCATATTACTCACCACATTATTATTATTATTATTATTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTAGGTGGTTGAATGGTGTTGCCATT |
PE14D | 7 | 413 | 114 | 130 | 115 | 133 | 136 | 121 | TGTAATGAATGATTCTAATACCAC | TTGGACCATGCTTCACAG | TGTAATGAATGATTCTAATACCACTACTACTACTACTACTACTACTACTACTACTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAACCTACTTCCTATTTGGATTTCTGTGAAGCATGGTCCAA |
TA127 | 8 | 1161 | 118 | 132 | 132 | 124 | 120 | 124 | GCTTTATAAAAATAACACACC | TAAAAAACACTCAGTTTGAG | GCTTTATAAAAATAACACACCTATATATAAATATATATATATATATATATATATATATTATATACATTTCTATCTTTATTCTTTCTTTTTATTTTTTTTTTTTATCTCAAACTGAGTGTTTTTTA |
C9108 | 9 | 1145 | 96 | 122 | 113 | 116 | 137 | 114 | AATATCTACACAACTAAAGC | CAAAAGAATCATCCTTTTCC | AATATCTACACAACTAAAGCATATACATATATATATTATATATATATATATATATATATATATATATATATATATATATATAATATATATGTATATATTTTTTTTTTTTTTTTAATAAATGGAAAAGGATGATTCTTTTG |
401780 | 10 | 402 | 103 | 80 | 80 | 105 | 90 | 86 | GAAACATAAAGGGATGTGTA | ACTTAGAAGAAATTCAATGC | GAAACATAAAGGGATGTGTATAAGTATATGTATATATATATATATATATATATATATATATATTTCTTATTGATCGCATTGAATTTCTTCTAAGT |
1451458 | 14 | 1452 | 215 | 203 | 203 | 194 | 205 | 209 | GAGCATTATAAAATTGGCTA | AAAAAGAAAAGAGATGAACA | GAGCATTATAAAATTGGCTACTAGTGAATATCTAAAATGTACATAAATAAATAAATAAATAAATAAATAAATATGTACATATATATATATATATATATATATATATCAAAATGTACAAAAATAGAATTTAAAGTATACTTTTATCTATCTGTACCAAATATGTGTTACATATTTATATATAATAATGTTCATCTCTTTTCTTTTT |
2549455 | 14 | 2550 | 178 | 174 | 179 | 176 | 179 | 175 | AAATATATGTTCTACTTTCAATCA | GAGGAAATAATATAATACACCA | AAATATATGTTCTACTTTCAATCATATAATAATTTTTTTTTTTTTTACTTATTAGTTTATAACTAAAAATTTTTCAATATATATATATATATATATATATATTCAGTACTCTATTAATTATAATTATTATATGTATCTATTTTTTATATTATTAATAGATGGTGTATTATATTATTTCCTC |