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Fig. 1 | Malaria Journal

Fig. 1

From: Humanized DRAGA mice immunized with Plasmodium falciparum sporozoites and chloroquine elicit protective pre-erythrocytic immunity

Fig. 1

CPS-CQ immunization alters the numbers of human T and B cells in DRAGA mice. DRAGA mice (n = 7) were immunized three times with CPS-CQ at 2 weeks interval, and 3 weeks after the last immunization the splenic cells and liver mononuclear cells were stained with human CD45, CD3, CD4, CD8, CD19, and FOXP3 antibodies and analysed by FACS on the mononuclear FSC/SSC gated cell population. Controls were untreated DRAGA mice (n = 8) or DRAGA mice treated with CQ alone (n = 2). a Frequency (upper panels) and total numbers (lower panels) of human CD45, CD4 and CD8 T cells, and B (CD19+) cells in spleens (left panels) and liver (right panels). b Frequency (upper panel) and total numbers (lower panel) of human CD4+FOXP3+ regulatory T cells (Treg) in the spleen and liver. Student t test p values for comparison of CPS-CQ (n = 7) and control mice (n = 8) in panels A&B are shown over the histograms. The mean average human cell values of two mice treated with CQ alone were similar to mice treated with CPS-CQ and reduced as compared to control mice. c Cytokine secretion by splenic and liver mononuclear cells from CPS-CQ immunized and control mice stimulated with PMA/ionomycin. Student t test p values are shown over the histograms

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