Fig. 6
From: PfCap380 as a marker for Plasmodium falciparum oocyst development in vivo and in vitro
Gametocytes do not express PfCap380. Purified GFP-Luc gametocytes and ookinetes were labelled with anti-PfCap380 antisera and co-labeled with antibodies against gametocyte (Pfs230, Pfs48/45) or ookinete (chitinase) markers. Gametocytes and ookinetes express GFP in green and nuclei stain with DAPI in blue. PfCap380 is shown in red; Pfs230 (a), Pfs48/45 (b), and chitinase (c) are in purple. The merged image of the four separate channels is shown in the bottom row. Gametocytes express Pfs230 and Pfs48/45 but not PfCap380. Ookinetes express chitinase but not PfCap380. Scale bars = 10 μm in a; 7.5 μm in b and c