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Fig. 1 | Malaria Journal

Fig. 1

From: Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages

Fig. 1

Evaluation of anti-His-PfNMNAT IgG antibodies on the recombinant protein. a 12% SDS-PAGE stained with Coomassie brilliant blue R-250, 1. 200 ng BSA; 2. 200 ng His-PfNMNAT; M. molecular weight marker (Thermo Scientific). b Western blot, polyvinylidene difluoride (PVDF) membrane, horseradish peroxidase (HRP) system. Unrelated serum murine (1:1000 dilution): 1. 125 ng BSA 2; 125 ng His-PfNMNAT. c Western blot, PVDF membrane, HRP system. Murine primary antibody (1:5000) for identification of recombinant His-PfNMNAT: 1. 125 ng BSA; 2–7. His-PfNMNAT: 2. 125 ng; 3. 60 ng; 4. 30 ng; 5. 15 ng; 6. 7 ng; 7. 3 ng

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