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Fig. 2 | Malaria Journal

Fig. 2

From: Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages

Fig. 2

Evaluation of anti-His-PfNMNAT IgY and IgG antibodies on Plasmodium falciparum asynchronous extracts. a 12% SDS-PAGE stained with Coomassie brilliant Blue R-250. b PVDF membrane. 1. Positive control: Recombinant protein MBP-PfNMNAT (66 kDa). 2. 80 µg of Plasmodium falciparum FCR3 extract from asynchronous culture. M. molecular weight marker (Thermo Scientific). The red arrow shows the identification of a protein of approximately 28 kDa corresponding to endogenous PfNMNAT. Primary antibody 1:1000 (pre-imune serum IgY, anti-His-PfNMNAT chicken serum IgY, anti-His-PfNMNAT egg yolk IgY, or murine blood IgG), secondary antibody 1:8000 (biotinylated anti-IgY or anti-IgG), streptavidin–alkaline phosphatase 1:8000, developed with the chromogenic 5-bromo-4-chloro-3′-indolyphosphate/nitro-blue tetrazolium (BCIP/NBT) substrate system

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Malaria Journal

ISSN: 1475-2875

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