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Fig. 3 | Malaria Journal

Fig. 3

From: Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stages

Fig. 3

PfNMNAT phosphorylation. PfNMNAT was immunoprecipitated using IgY antibodies obtained from chicken serum and thiophilic resin. A–E 15 μl of the immunoprecipitated product A. 12% SDS-PAGE silver-stained. B–E PVDF membrane. B Primary antibody 1:1000 anti-His-PfNMNAT IgG. C Anti-T-phosphorylated IgG. D Anti-Y-phosphorylated IgG. E Anti-S-phosphorylated IgG. Secondary antibody 1:8000 (biotinylated anti-IgY or anti-IgG), streptavidin–alkaline phosphatase 1:8000, developed with the BCIP/NBT chromogenic substrate system

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Malaria Journal

ISSN: 1475-2875

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