Fig. 4

Immunolocalization of PfNMNAT at different stages of the asexual life cycle of Plasmodium falciparum. Primary antibody: anti-His-PfNMNAT IgY serum (1:250). Unrelated serum (1:250). Secondary anti-α-FITC IgY antibodies (1:1000). Nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/ml). The images were taken at ×100 magnification using a Nikon C1 plus confocal fluorescence microscope