Fig. 6From: Localization and phosphorylation of Plasmodium falciparum nicotinamide/nicotinate mononucleotide adenylyltransferase (PfNMNAT) in intraerythrocytic stagesProtein expression analysis of PfNMNAT. 150 μg protein from the ring (R), trophozoite (T) and schizont (S) stages were loaded. As a positive control, 150 ng of recombinant protein MBP-PfNMNAT (66 kDa) was loaded (1). M. molecular weight marker (Thermo Scientific). 12% SDS-PAGE stained with Coomassie Blue R-250 and western blot on a PVDF membrane. Primary anti-His-PfNMNAT IgY antibody (1:1000). Secondary antibody: biotinylated anti-chicken (1:8000). Streptavidin–alkaline phosphatase (1:8000). Developed with the BCIP/NBT substrate systemBack to article page