Fig. 4

Measures of sequence diversity for PvDBP and relationship to key binding interfaces. a Spatially-derived nucleotide diversity (π) for PvDBP displayed over the modelled PvDBP structure. A radius of 15 Å was used for each 3D sliding window. b Normalized Shannon entropy for PvDBP residues on a per-site basis, with no spatial averaging performed. Higher entropy values are indicative of greater sequence diversity across all strains at that residue position. c Residues involved in the PvDBP dimerization and DARC binding interface. Residues within 4 Å of the corresponding dimer chain or the DARC ligand (as defined by the PDB structure 4NUU) are shown in blue on the modelled PvDBP structure