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Table 1 Summary of the results obtained for the multiplex strategies by mixing simplex reactions

From: A novel field-based molecular assay to detect validated artemisinin-resistant k13 mutants

  1. * Of note, similar performances with those selected were obtained with 446/580 and IC/539 duplexes, demonstrating the multiplexing flexibility of the ARMS strategy
  2. Failed means: a decrease of sensitivity to detect both SNPs, b presence of non-specific amplification curves, c decrease of sensitivity to detect one SNP (R539T), d overlap of the primers set