Multiplicity and molecular epidemiology of Plasmodium vivax and Plasmodium falciparum infections in East Africa

Table 2 Comparison of three methods for determination of multiplicity of infection (MOI) of P. vivax: long fragment (422 bp) of pvmsp1 amplicon deep sequencing, short fragment (117 bp) of pvmsp1 amplicon deep sequencing, and microsatellite marker genotyping

	Amplicon deep sequencing Long fragment (422 bp)	Amplicon deep sequencing Short fragment (117 bp)	Microsatellite markers^a
Number of subject	135	135	58
Median MOI	2	1	1
Mean MOI^a	2.16^a	1.64^b	1.07^c
Max MOI	6	4	3
No. polyclonal	84	62	3
% polyclonal	62.2	45.9	5.2
No. alleles	88	29	24
Heterozygosity (He)	0.92	0.84	0.77

Significant differences were detected in mean MOI among the three methods as indicated by the superscripts (Tukey–Kramer HSD test, P < 0.05)
He: Expected heterozygosity corrected for sample size
^aRefer to Lo et al. (2015) [39]

ISSN: 1475-2875