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Table 1 Nucleotide sequences of the LAMP primers constructed for kelch13 of Plasmodium falciparum

From: An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer

Primer

Sequence (5′–3′)

Length

LAMP primers

 F3

TGGGGGATATGATGGCTC

18

 B3

ATTATCAATACCTCCAACAACAT

23

 FIP

AGCTGATGATCTAGGGGTATTCAA-TTCTATTATACCGAATGTAGAAGCA

49

 BIP

AATGGGAACAATTTCCATATGCCT-GATTAAGGTAATTAAAAGCTGCTC

48

 LF

CCCATGCTTTCATACGATGATCATA

25

  1. F3 and B3, outer primers; FIP and BIP, inner primers; LF, loop primer
  2. The FIP primer consisted of F2 and the complementary strand (F1c)
  3. The BIP primer consisted of B2 and the complementary strand (B1c)
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Malaria Journal

ISSN: 1475-2875

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