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Table 2 Summary of the results of nested PCR and LAMP amplifications, and the identification of kelch13 mutations of P. falciparum

From: An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer

 

LAMP reaction

Identification of mutation

PCR and Sanger

LAMP and MinION

Positive

Negative

C580Y

Wild type

C580Y

Wild type

Nested PCR

 P. f

33

33

0

0

33

0

33

 P. f/P. v

1

1

0

0

1

0

1

 P. v

6

0

6

–

–

–

–

  1. P. f, P. falciparum; P. v, P. vivax; P.f/P.v, P. falciparum and P. vivax co-infection