Fig. 3

Repeatability and intermediate precision of sample measurements. IgG in samples from malaria-naïve (non-exposed) and malaria-exposed individuals from an IPTp clinical trial in Mozambique, were measured in triplicates on 22 different days against 7 P. falciparum antigens. a Repeatability represented by boxplots of log₁₀ MFI distributions for all antigens together at each repetition (r1, r2, r3); b boxplots of coefficient of variation between replicates by antigen and sample type. c Intermediate precision represented by boxplots of log₁₀ MFI distribution for all antigens together at each day of analysis, and d boxplots of coefficient of variation between days of analysis by antigen and sample type. Boxplot horizontal lines represent median and interquartile range. e Bland–Altman plots showing the difference of each day versus the mean of all days of analysis for each antigen and both sample types