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Table 2 Parameters from the non-linear 4-parameter logistic (4-PL) regression function for singleplex curves

From: Development of a high-throughput flexible quantitative suspension array assay for IgG against multiple Plasmodium falciparum antigens

Analyte

Parameters

Coefficient

Standard error

95% CI

 

IgG

Emax

30,929.13

286.52

30,280.97

31,577.28

Emin

423.37

420.67

− 528.26

1375.00

EC50

3.53

0.15

3.18

3.88

Hill

1.41

0.08

1.23

1.60

mCSP

Emax

32,000.25

3650.55

23,742.15

40,258.36

Emin

4000.20

1547.16

500.27

7500.13

EC50

20.44

7.90

2.58

38.30

Hill

0.87

0.27

0.26

1.48

  1. Anti-mouse IgG-coupled beads incubated with purified mouse IgG and CSP-coupled beads incubated with mouse anti-CSP monoclonal antibody (mCSP) at known IgG concentrations
  2. CI confidence interval