Table 1 Genes expressed more or less than tenfold (p < 0.01) in the liver of vaccinated mice infected with P. chabaudi on day 4 p.i. (Vd4) in comparison to constitutive expression on day 0 p.i. (Vd0)
Gene | Gene description | RefSeq ID | Vd4 vs. Vd0 | p value | Function (annotated according to www.genecards.org) |
|---|---|---|---|---|---|
Erythropoiesis | |||||
 Ahsp | Alpha hemoglobin stabilizing protein | NM_133245 | 48.18 | 0.0095 | Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia |
 Cldn13 | Claudin 13 | NM_020504 | 43.50 | 0.0070 | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity |
 Ermap | Erythroblast membrane-associated protein | NM_013848 | 21.59 | 0.0036 | The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor, possibly as a mediator of cell adhesion |
 Gata1 | GATA binding protein 1 | NM_008089 | 29.73 | 0.0015 | Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development |
 Gfi1b | Growth factor independent 1B | NM_008114 | 20.50 | 0.0092 | Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages |
 Kel | Kell blood group | NM_032540 | 65.89 | 0.0010 | This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen |
 Rhag | Rhesus blood group-associated A glycoprotein | NM_011269 | 17.37 | 0.0001 | The protein encoded by this gene is erythrocyte-specific and is thought to be part of a membrane channel that transports ammonium and carbon dioxide across the blood cell membrane |
 Slc4a1 | Solute carrier family 4 | NM_011403 | 11.74 | 0.0067 | Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin |
Cell cycle and mitosis | |||||
 Ccnb1 | Cyclin B1 | NM_172301 | 17.76 | < 0.0001 | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF) |
 Cdc25c | Cell division cycle 25C | NM_009860 | 12.41 | 0.0009 | Cdc25 activates cdk complexes that drive the cell cycle. Cdc25 is involved in the DNA damage checkpoints and is known as a key mediator of cell cycle progression |
 Ckap2 l | Cytoskeleton associated protein 2-like | NM_181589 | 10.72 | 0.0030 | Microtubule-associated protein required for mitotic spindle formation and cell-cycle progression in neural progenitor cells |
Innate immunity | |||||
 Abcg4 | ATP-binding cassette, sub-family G (WHITE), member 4 | NM_138955 | 26.79 | 0.0035 | The protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. May be involved in macrophage lipid homeostasis |
 Ccl7 | Chemokine (C-C motif) ligand 7 | NM_013654 | 34.97 | 0.0013 | Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils |
 Socs1 | Suppressor of cytokine signaling 1 | NM_009896 | 11.32 | 0.0070 | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma |
Treml2 | Triggering receptor expressed on myeloid cells-like 2 | NM_001033405 | 10.92 | 0.0001 | Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation |
 Cd163 | CD163 antigen | NM_053094 | 0.03 | 0.0018 | The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage |
Cytotoxicity | |||||
 Gzmb | Granzyme B | NM_013542 | 35.66 | 0.0085 | The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing |
 Klrb1a | Killer cell lectin-like receptor subfamily B member 1A | NM_010737 | 10.43 | 0.0083 | Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3 |
 Klrc3 | Killer cell lectin-like receptor subfamily C, member 3 | NM_021378 | 11.38 | 0.0002 | KLRC3 is a member of the NKG2 group which are expressed primarily in natural killer (NK) cells and encodes a family of transmembrane proteins characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain |
 Klrd1 | Killer cell lectin-like receptor, subfamily D, member 1 | NM_010654 | 16.33 | 0.0067 | Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells |
 Ncr1 | Natural cytotoxicity triggering receptor 1 | NM_010746 | 16.97 | 0.0084 | Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis |
Miscellaneous | |||||
 Hist1h3g | Histone cluster 1, H3g | NM_145073 | 18.90 | 0.0075 | Core component of nucleosome |
 Htr7 | 5-Hydroxytryptamine (serotonin) receptor 7 | NM_008315 | 12.53 | < 0.0001 | Serotonin 5-HT7 receptors are located primarily in the thalamus, hypothalamus and hippocampus. The function of these receptors includes the regulation of circadian rhythms, thermoregulation, learning and memory and smooth muscle relaxation |
 Nxpe5 | Neurexophilin and PC-esterase domain family, member 5 | NM_001013773 | 22.61 | 0.0089 | Unknown |