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Fig. 4 | Malaria Journal

Fig. 4

From: Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies

Fig. 4

Levels of IgG1 measured to 15 antigens in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific IgG1 levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h, 4 °C overnight (4 °C ON) and room temperature 1 h (RT 1 h). “neg” means negative control

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