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Fig. 2 | Malaria Journal

Fig. 2

From: Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

Fig. 2

Droplet digital PCR for oocyst genome quantification. a One-dimensional scatter plots showing 18S ddPCR assay on positive (syn18s standards from 7.2 × 104 to 0.72 copies/µL) and negative human blood (“HGD”) extracts. Clear demarcation between positive and negative partitions is shown. Uninfected human blood extract reaction was used to determine a universal positive/negative threshold set at 1853. b Quantification of syn18s standards using ddPCR in triplicate (black circles indicate the median of each run with error bars showing 95% CI) compared to the predicted qPCR value (grey line). c One-dimensional scatter plots showing 18S ddPCR assay on 14 oocyst-positive midguts, with positive and negative partitions. Uninfected mosquito midguts (“Neg”) were used to determine a universal positive/negative threshold set at 1853. d Quantification of genomes per oocyst for the14 microscopy-confirmed oocyst-positive midguts using 18S qPCR and 18S ddPCR. Box plots indicate the median and whiskers show the minimum and maximum responses. Groups compared using Wilcoxon matched-pairs signed rank test (p = 0.43)

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