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Table 2 Manual extraction efficiency evaluated using EHV and RPS7 markers

From: Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

PCR target

Midguts from mosquitoes fed on gametocyte culture

Mean Cp (95% CI)

Midguts with microscopy-confirmed P. falciparum oocysts

Mean Cp (95% CI)

EHV

24.75

(24.68–24.82)

24.14

(23.68–24.61)

RPS7

22.85

(22.68–23.02)

23.03

(22.0–24.07)

  1. Mean Cp and 95% CI values displayed for the EHV and RPS7 PCR analysis of An. stephensi mosquito midguts fed on gametocyte culture with unknown infection status (n = 96) or with microscopy-confirmed P. falciparum oocysts (n = 11/oocyst numbers range 1–4). The 11 microscopy-positive midguts were also confirmed positive by 18S qPCR
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Malaria Journal

ISSN: 1475-2875

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