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Table 4 18S ddPCR with synthetic DNA standards

From: Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

 

qPCR

ddPCR (DNA copies/µL)

qPCR Cpa

Predicted DNA copies/µLb

Totalc

Individual meanc (95% CId)

syn18s-5

19.5

72,100

Saturated

Saturated (n/a)

syn18s-6

22.7

7210

6176

6198.7 (6790–5600)

syn18s-7

26.1

721

714

717.3 (804–628)

syn18s-8

29.4

72

72

72.4 (83.2–61.6)

syn18s-9

33.3

7.2

6.6

6.7 (8.1–8.3)

syn18s-10

36.7

0.72

0.84

0.9 (1.5–0.4)

  1. a qPCR Cp values for the synthetic standards are shown as means (n = 18). No quantification results produced for qPCR as the synthetic standards were used to produce standard curve
  2. b syn18s DNA predicted copies/µL as calculated based on Qubit reading
  3. c Triplicate ddPCR reaction data for each dilution is shown as total and individual amounts, where the total amount was calculated based on the triplicate reaction data being treated as one larger sample
  4. d ddPCR 95% confidence intervals (CI) are shown based on the total data calculations
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Malaria Journal

ISSN: 1475-2875

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