Fig. 6

DNA hydrolytic test of the five GST-tagged recombinant proteins with different divalent metals. Divalent metal ions at different concentrations were added into the reaction and incubated for 30 min. The lane with no protein is the blank control with only genomic DNA incubated for 30 min. a No specific effect of divalent metal ions on the activity of GST-PF3D7_1238600 was observed. b The activity of GST-PF3D7_0107200 was dependent on Cu²⁺ with an optimal concentration of 2 mM. c The activity of GST-PF3D7_0305600 was dependent on Mn²⁺ and Mg²⁺. d The activity of GST-PF3D7_1363500 was dependent on Cu²⁺ with an optimal concentration of 2 mM. e The activity of GST-PF3D7_0319200 was dependent on Cu²⁺ with an optimal concentration of 10 mM