Table 2 Primers in construction of His-tagged recombinant proteins and expression plasmid
From: Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone
Genes ID | Primers | Recombinant plasmid | |
|---|---|---|---|
PF3D7_1238600 | Forward primer | GGATCC TATGATATTGATATATTAGTTCT | pET-28a |
Reverse primer | CTCGAG ATTTGCAACTAGGATATAATCTA | ||
PF3D7_0107200 | Forward primer | GGATCC TTTAATAGCGCTTGGTATACA | pET-28a |
Reverse primer | CTCGAG ATGATCTGATGGGAAATGCTC | ||
PF3D7_1363500 | Forward primer | GGATCC ATACCTTCTGTGCGTAATATAAA | pET-28a |
Reverse primer | CTCGAG TAAGAGCTCAAATACTTTGG | ||
PF3D7_0305600 | Forward primer | GGATCC AATAATAATTCTCCCTTTAGTCA | pET-28a |
Reverse primer | CTCGAG TAAACTAAAATAAGCATTATAATC | ||
PF3D7_0319200 | Forward primer | GGATCC AAAAGTTATACATTTCCATATATA | pET-28a |
Reverse primer | TCTGAG TAAAAGCTTTAATTCCTTGTGAT | ||