Table 3 Primers in construction of GST-tagged recombinant proteins and expression plasmid
From: Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone
Genes ID | Primers | Recombinant plasmid | |
|---|---|---|---|
PF3D7_1238600 | Forward primer | GGATCC TATGATATTGATATATTAGTTCT | pGEX-4T-1 |
Reverse primer | CTCGAG ATTTGCAACTAGGATATAATCTA | ||
PF3D7_0107200 | Forward primer | GGATCC TTTAATAGCGCTTGGTATACA | pGEX-4T-1 |
Reverse primer | CTCGAG ATGATCTGATGGGAAATGCTC | ||
PF3D7_1363500 | Forward primer | GGATCC AAGGAATTTTCCGTTTTCTCTTT | pGEX-4T-1 |
Reverse primer | CTCGAG GTACGAATAAAATATATAATCTACAC | ||
PF3D7_0305600 | Forward primer | GGATCC ATTATTGTTACATGGAATATGAAT | pGEX-4T-1 |
Reverse primer | CTCGAG TTCATTTTTGAGGTATAATATAAC | ||
PF3D7_0319200 | Forward primer | GGATCC CGTATTCTATCGTATAATATTTTAGCA | pGEX-4T-1 |
Reverse primer | CTCGAG TAATTCTACCTCAGCAGCTATG | ||