Fig. 3

Natural extract screening for anti-malarial activity using 3D7 strain. a Plasmodium falciparum 3D7 strain-infected human RBCs were incubated with 100 µg/ml test extracts (120 different extracts) for 96 h (see “Methods”). Nineteen extracts showed > 90% growth inhibitory effects. b Three selected extracts (Caryophylli flos, Coptidis rhizoma, and Phellodendri cortex) were investigated further for concentration-dependent growth inhibition effects. Different concentrations of each extract were incubated with parasites for 96 h. Concentration-dependent anti-malarial effects were observed and IC₅₀ values were calculated (see Table 2)