Fig. 2
Verification of the integration of the F446I mutation into the target locus. a PCR amplification using the indicated primers produced the expected fragments in lanes 4, 5, 7, and 8 for the transgenic parasite lines, whereas no fragments were detected in the other lanes for the parent parasites. Lanes 1–9: negative control, pARL-K13F446I plasmid, pARL-K13C580Y plasmid, FCC1/HNC580Y mut, FCC1/HNF446I mut, FCC1/HN wt, 3D7C580Y mut, 3D7F446I mut and 3D7wt. b PCR analysis showed that the expected fragments were amplified from the transgenic parasites. Lanes 1–9, as indicated above. c Sequencing verification of the mutations from T to A for F446I and G to A for C580Y