Fig. 3

Verification of only k13 with the F446I mutation expressed. a Analysis of expression of both the wild-type and mutant k13 alleles from the 3D7F446Imut parasite. The gel blot shows the K13 gene amplified from the RNA extracted from the transgenic parasite (lane 1, no-RT control) and the cDNA (lane 2); sequence analysis shows all the 12 clones have the F446I mutant alleles. b Western blot analysis of PfK13 expression in the transgenic parasite lines and their parent strains