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Table 1 Primer sequences for LAMP amplification of the Plasmodium genus for detection of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale and Plasmodium malariae

From: Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination

Species

Primer

Primer sequence (5′ to 3′)

P. falciparum, P. vivax, P. ovale, P. malariae)

Forward Inner Primer (F1P) (F1c + F2 regions)

AGCTGGAATTACCGCGGCTGGGTTCCTAGAGAAACAATTGG

Backward Inner Primer (B1P) (B1-B2c regions)

TGTTGCAGTTAAAACGTTCGTAGCCCAAACCAGTTTAAATGAAAC

Forward Outer Primer (F3) (F3c + F3 region)

TGTAATTGGAATGATAGGAATTTA

Backward Outer Primer (B3) (B3 + B3c region)

GAAAACCTTATTTTGAACAAAGC

Loop Forward Primer (LPF)

GCACCAGACTTGCCCT

Loop Backward Primer (LPB)

TTGAATATTAAAGAA