Fig. 3

Evaluation of optimized immune complex dissociation protocol and pull-down assay in mock patient samples. a In the absence of α-HRP2 antibodies, no HRP2 was detectable in the immune complex pull-down assay. Additionally, heating free HRP2 did not diminish ELISA signal. b Increasing equivalents of α-HRP2 C1–13 decreased ELISA signal, but heating fully restored that signal. c Immune complex pull-down recovered 60% of complexed HRP2 over a wide range of parasite concentrations