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Fig. 1 | Malaria Journal

Fig. 1

From: Immunological characterization of Plasmodium vivax Pv32, a novel predicted GPI-anchored merozoite surface protein

Fig. 1

Schematic diagram of Pv32, recombinant Pv32 protein expression and immunization. a Schematic diagram of Pv32. The Pv32 protein comprises 287 amino acids, with a calculated molecular mass of 32 kDa. The signal peptide (amino acid [aa] position 1–22) and GPI motif (aa, 270–287) are indicated. Truncated Pv32 (23–269 aa) was constructed for expression. The red bar indicates the cysteine residue position. SP signal peptide, GPI glycosylphosphatidylinositol. b The purification progress of Pv32 (58 kDa) was resolved by 12% SDS-PAGE. T total, S supernatant, P pellet, Ft flow-through, E elution. c Western blot analysis of recombinant Pv32 with anti-GST antibody (GST), rabbit immune sera (R), pooled P. vivax patient sera (P), PBS-immunized rabbit sera (NR), and non-infected human sera (H). Arrowheads indicate specific bands for each recombinant protein

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