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Fig. 1 | Malaria Journal

Fig. 1

From: Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood

Fig. 1

Plasmodium yoelii 18S rRNA versus 18S rDNA in blood collected from the tail vein of BALB/cj mice after tail vein inoculation of sporozoites. a Plasmodium yoelii sporozoites were injected by the tail vein and then whole blood was collected onto dried blood spots at the indicated hour post-inoculation, preserved in lysis buffer and extracted for total nucleic acids. qPCR or qRT-PCR was performed and the CTs are plotted. Red squares: qRT-PCR (18S rDNA + 18SrRNA), Black circles: qPCR (18S rDNA). Dashed box indicates 5 × 104 freshly-dissected sporozoites (Spz). Each data point represents a unique blood sample obtained from an individual animal at the stated time point. b Using the standard curve of diluted parasites, the log10 copies/mL difference between 18S rRNA and 18S rDNA was calculated for each time point for each mouse

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