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Table 1 Summary of experiments to address route-specific Plasmodium 18S rRNA positivity in mouse studies

From: Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood

Mouse studies 18S rRNA (# positive)a 18S rRNA CT (range) 18S rDNA (# positive) 18S rDNA CT (range) ΔC bT (range)
Tail vein inoculation/tail vein sampling experiment
 Immediate 3/3 31.21–33.48 3/3 35.56–36.93 2.08 to 5.73
 0.2 h 3/5 28.84–35.09 5/5 34.91–36.30 0.81 to 6.18
 0.5 h 1/5 31.35–35.22 5/5 35.14–36.05 0.14 to 3.79
 1 h 0/5 34.33–36.20 5/5 35.22–36.20 − 0.99 to 1.03
 24 h 0/5 34.87–36.55 5/5 35.10–36.13 − 0.42 to 0.84
 48 h 5/5 14.05–19.16 5/5 28.56–32.80 13.64 to 14.51
Retro-orbital inoculation/cardiac puncture sampling experiment
 24 h 0/3 N/A 0/3 N/A N/A
  1. Tail vein (3–5 μL dried blood spots); cardiac puncture (50 μL whole blood)
  2. N/A not applicable
  3. a18S rRNA positivity defined as a ≥ 2-fold relative increase in 18S rRNA compared to rDNA
  4. bΔCT equals the CT for the qPCR (rDNA) minus the CT for the qRT-PCR (rRNA) for an individual sample irrespective of positivity as described above such that a high ΔCT indicates a large amount of 18S rRNA relative to the coding DNA