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Table 1 Summary of experiments to address route-specific Plasmodium 18S rRNA positivity in mouse studies

From: Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood

Mouse studies

18S rRNA (# positive)a

18S rRNA CT (range)

18S rDNA (# positive)

18S rDNA CT (range)

ΔC bT (range)

Tail vein inoculation/tail vein sampling experiment

 Immediate

3/3

31.21–33.48

3/3

35.56–36.93

2.08 to 5.73

 0.2 h

3/5

28.84–35.09

5/5

34.91–36.30

0.81 to 6.18

 0.5 h

1/5

31.35–35.22

5/5

35.14–36.05

0.14 to 3.79

 1 h

0/5

34.33–36.20

5/5

35.22–36.20

− 0.99 to 1.03

 24 h

0/5

34.87–36.55

5/5

35.10–36.13

− 0.42 to 0.84

 48 h

5/5

14.05–19.16

5/5

28.56–32.80

13.64 to 14.51

Retro-orbital inoculation/cardiac puncture sampling experiment

 24 h

0/3

N/A

0/3

N/A

N/A

  1. Tail vein (3–5 μL dried blood spots); cardiac puncture (50 μL whole blood)
  2. N/A not applicable
  3. a18S rRNA positivity defined as a ≥ 2-fold relative increase in 18S rRNA compared to rDNA
  4. bΔCT equals the CT for the qPCR (rDNA) minus the CT for the qRT-PCR (rRNA) for an individual sample irrespective of positivity as described above such that a high ΔCT indicates a large amount of 18S rRNA relative to the coding DNA