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Fig. 3 | Malaria Journal

Fig. 3

From: Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Fig. 3

PvCSP(VK210) and PvCSP(VK247) expression in oocyst-derived sporozoites of two chimeric P. falciparum parasite lines (pf-pvcsp). Immunofluorescence analyses of wild type P. falciparum (WT) sporozoites and oocyst-derived pf-pvcsp sporozoites. Fixed sporozoites were labelled with mouse anti-PvCSP-VK210 mAb, anti-PvCSP-VK247mAb and mouse anti-PfCSP antibodies. As a control an antibody against PfHSP70 was used. Secondary conjugated antibodies used: anti-IgG Alexa Fluor® 488 (green) or anti-IgG Alexa Fluor ® 594 (red). Nuclei stained with the DNA-specific dye Hoechst-33342. All pictures were recorded with standardized exposure/gain times; Alexa Fluor® 488 (green) 0.7 s; anti-IgG Alexa Fluor ® 594 (red) 0.6 s; Hoechst (blue) 0.136 s; bright field 0.62 s (1× gain). Scale bar, 7 µm

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Malaria Journal

ISSN: 1475-2875

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