Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Table 1 Gametocyte, oocyst and sporozoite production in WT, pf-pvcsp(vk210), pf-pvcsp(vk247) and PfΔcsp

Lines	No of gametocytes stage V male/female mean (SD)^a	No. of exflagellation mean (SD)^b	No. of oocyst mean (range)^c	No of sporozoites^d
WT
NF54 (n = 4)	m: 0.6 (0.2) f: 1.3 (0.3) (3 exp.)	1436 (191) (3 exp.)	28.2 (13–54) (4 exp)	9–20 K (4 exp)
pf - *pvcs* (vk210)
0050cl7 (n = 5)	m: 0.6; f: 1.1 (1 exp.)	2030 (916) (3 exp.)	44.2 (30–63) (5 exp)	Negative
0050cl2 (n = 1)	ND	ND	9.1 (1 exp.)	Negative
pf - *pvcs* (vk247)
0041cl5 (n = 3)	m: 0.8; f:1.5 (1 exp.)	460 (34) (3 exp.)	23.1 (17–40) (3 exp.)	Negative
Pf Δ *csp*
0113cl1 (n = 1)	ND	875 (1 exp.)	4.6	Negative
0113cl3 (n = 2)	m: 0.6 f: 1.1 (1 exp.)	1010 (388) (3 exp.)	2.2 and 3.2 (2 exp.)	Negative

^aMean percentage of stage V male (m) and female (f) gametocytes (per 100 red blood cells) in day 14 cultures in 2–7 experiments (exp.)
^bMean number of exflagellating male gametocytes (per 10⁵ red blood cells) at 10–20 min after activation of day 14 gametocyte cultures (sd standard deviation)
^cMean number of oocyst per mosquito at day 9–10 after feeding. Range corresponds to the mean number of retorts in multiple experiments (1–5 exp. per line; 10–30 mosquitoes per exp.)
^dMean number of salivary gland sporozoites per mosquito at day 21 after feeding. Range corresponds to the mean number of sporozoites in multiple experiments (1–5 exp. per line; 20–30 mosquitoes per exp.)

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