Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Marin-Mogollon, Catherin; van Pul, Fiona J. A.; Miyazaki, Shinya; Imai, Takashi; Ramesar, Jai; Salman, Ahmed M.; Winkel, Beatrice M. F.; Othman, Ahmad Syibli; Kroeze, Hans; Chevalley-Maurel, Severine; Reyes-Sandoval, Arturo; Roestenberg, Meta; Franke-Fayard, Blandine; Janse, Chris J.; Khan, Shahid M.

doi:10.1186/s12936-018-2431-1

Table 2 Parasite lines of different Plasmodium species expressing heterologous or mutated CSP and mutants lacking CSP expression

From: Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Plasmodium species	CSP^a	Oocyst no.^b	Salivary gland sporozoite no.^b	Reference; RMgmDB ID^c	Remarks (mutation, sporozoite phenotype)
Chimeric CSP parasite lines
P. berghei	Py CSP	WT	WT	[44]; 75
P. berghei	Pf CSP	WT	Reduced (90%)	[52]; 69
P. berghei	Pf CSP	WT	Reduced (90%)	342
P. berghei	Pf CSP	WT	WT	[53]; 4110
P. berghei	Pf CSP	WT	WT	[54]; 4135
P. yoelii	Pf CSP	WT	WT	[55]; 1442
P. berghei	Pv CSP-210	WT	WT	[27]; 4136
P. berghei	Pv CSP-247	WT	WT	[28]; 1443
P. berghei	Pv CSP-247	WT	Reduced (30%)	[27]; 4137
P. berghei	Pg CSP	WT	Absent	[44]; 74	WT oocyst sporozoite formation
P. falciparum	Pv CSP-210	WT	Absent	This study	Sporozoite formation in fraction of oocysts
P. falciparum	Pv CSP-247	WT	Absent	This study	Sporozoite formation in fraction of oocysts
Knock-out CSP parasite lines
P. berghei	–	WT	Absent	[42]; 9	No sporozoite formation
P. knowlesi	–	WT	Absent	[43]	No sporozoite formation
P. falciparum	–	WT	Absent	This study	No sporozoite formation
Mutated CSP parasite lines (with a sporozoite production phenotype)
P. berghei	Mut. Pb CSP	WT	Absent	[45]; 72	PbCSP with truncated 3′UTR; reduced sporozoite formation in oocysts
P. berghei	Mut. Pb CSP	WT	Absent	[50]; 73	Mutations of the C-terminal GPI-anchor. No sporozoite formation in oocysts
P. berghei	Mut. Pb CSP	WT	Absent	[46]; 1148	PbCSP lacking repeat region; reduced sporozoite formation in oocysts; no midgut/salivary gland sporozoites
P. berghei	Mut. Pb CSP	WT	Absent	[46]; 1149	PbCSP lacking repeat region and NH2 terminus; no sporozoite formation in oocysts
P. berghei	Pb/Pg CSP	WT	Absent	[56]; 770	pbcsp replaced by pgcsp with repeat region of pbcsp

^aExpression of heterologous CSP or mutated CSP
^bOocyst numbers and salivary gland sporozoite numbers in infected An. stehensi mosquitoes compared to wild type (WT) infected mosquitoes. WT numbers in the same range as WT-infected mosquitoes
^cMutant ID in the RMgmDB database: https://www.pberghei.eu

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