Table 2 PCR primers used for this study
Oligo name | Sequence | Â |
|---|---|---|
Primers used for Genotyping of the GAP MCB | ||
 mo517 | GGAACTCATTAATATGTATG |  |
 mo518 | CAAAACCCATACTAAAAAAG |  |
 mo519 | GAGAACTTTAGCACAAAAGC |  |
 mo520 | TTTACGCTTTCTGCATCTTC |  |
 Aw171 | CCTAATCATGTAAATCTTAAATTTTTC |  |
 Aw560 | CCAATAGAT AAAATTTGTAG |  |
Oligo name | Sequence | Target |
|---|---|---|
Primers used for qRT PCR | ||
 KAHRP-FB | CATATAGTGCAATAATGGAAACGGA | Pf KAHRP gene (inside excised region) |
 KAHRP-RB | GGTGATTTACTTCTCCATGATGATG | |
 KAHRP-F | TGTTCCAGCAGATGCACCAA | Pf KAHRP gene (in remaining region) |
 KAHRP-R | GAGCTGAATAGCCTGCACCA | |
 MDR1-T1 F | TATGCATTTGTGGGAGAATCAG | Pf MDR1 [multi drug resistance] (single copy gene reference) |
 MDR1-T1R | CTCCTTCGGTTGGATCATAAAG | |
 hDHFR (FG) | ATGCATGGTTCGCTAAACT | hDHFR [human dihydrofolate reductase] gene |
 hDHFR R1 | CCAGGTCTTCTTACCCATAAT | |
 hDHFR FA | CCTAATAGAAATATATCAGGATCCAT | hDHFR (recombination region) |
 hDHFR1 R | GGTCTTCTTACCCATAATCA | |
 LDH-T1F | AGGACAATATGGACACTCCGAT | Pf LDH1[lactate dehydrogenase] (single copy gene reference) |
 LDH-T1R | TTTCAGCTATGGCTTCATCAAA | |
 Pf SARS F1 | AAGTAGCAGGTCATCGTG | Pf SARS [seryl-tRNA synthetase] (housekeeping gene) |
 Pf SARS R1 | CGGCACATTCTTCCATA | |