Fig. 3

Statistical analysis of SMFA data. a Raw data for the number of oocysts per fed mosquito. These oocyst intensities were compared using a zero-inflated Poisson regression to accommodate the distribution of the count data. A Bayesian approach was used to demonstrate there is no statistical difference between experimental replicates. Zero-inflated Poisson probability distributions were fitted using Hamiltonian Monte Carlo sampling methods described in the main manuscript. There is a probability θ of drawing a zero and a probability (1 − θ) of drawing from a Poisson distribution with mean parameter λ. Four chains were initialised to assess the convergence of 2000 iterations, the first 1000 were discarded as burn in. The posterior distributions of parameters (4000 iterations) b θ and c λ for each experimental replicate (columns 1–3 respectively) are shown, posterior checks demonstrate the oocyst counts are not statistically different when mosquitoes feed on a glass (blue) or a 3D-printed (red) standard membrane feeding assay (SMFA)