Fig. 1

Parasite proteins associated with endothelial binding. Synchronized C24 and ItG parasites grown to mature stage (approximately 25–30 h post-invasion) were enriched by Plasmagel flotation and metabolically labelled using ³⁵S methionine. The labelled IE were used intact in co-culture with confluent TNF-activated HUVEC cells for 30 min after which unbound IE were removed and proteins prepared from the adherent cells. The figure shows autoradiographs of samples run on IEF gels (pH 4–7) followed by 12.5% SDS-PAGE co-culture with intact C24 infected erythrocytes with HUVEC (left) and intact ItG infected erythrocytes with HUVEC (right). Corresponding gels stained with Coomassie blue were used to ensure equivalent loading (data not shown). Scale on left hand side in kDa